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Gram Negative Bacteria: Pseudomonas aeruginosa and Escherichia coli


Gram Negative Bacteria: Pseudomonas aeruginosa and Escherichia coli

They are a lot! But don't worry, with ATP we will get to know gram negative bacteria one by one. In this video, we will be learning about two important gram negative bacilli: P. aeruginosa, and E. coli. We hope you enjoy and get the most out of the video!

0:12 - P. aeruginosa
0:46 - P. aeruginosa virulence factors
1:20 - Clinical importance
2:00 - E. coli
2:53 - E. coli virulence factors
4:26 - Clinical importance (and E. coli types)
4:47 - Enterotoxigenic E. coli (ETEC)
5:28 - Enterohemorrhagic E. coli (EHEC)
5:54 - Clinical importance Continued

Pseudomonas aeruginosa

What is Pseudomonas aeruginosa? Pseudomonas aeruginosa, or P. aeruginosa, is a gram-negative bacterium that is abundant in the environment.

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Gram-negative Bacilli

Escherichia coli, Klebsiella, Salmonella, Shigella, Haemophilus, Bordetella, Pseudomonas Aeruginosa, Pleomorphic Proteus, Helicobacter Pylori and Bacteroides

Enterobacteriaceae :

Questions and Answers segment is right after the lecture.

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Gram Negative Bacteria: Pseudomonas aeruginosa and Escherichia coli

Microbiology- bacteria, gram negative part 1

Antibiotics for Gram Negative Infections (Antibiotics - Lecture 5)

A summary of antibiotics used to fight infections caused by gram negative organisms, with a focus on Pseudomonas, ESBL, Acinetobacter, and Stenotrophomonas.

Gram negative bacteria : Escherichia coli (E. Coli)

This video is for isolation and identification Escherichia coli in Pharmaceutical companies, food factories and medical laboratory ????

Video includes gram staining, ability of movement by flagella (motile), main biochemical tests, growth on media agar on selective media and differential medium for isolation and identification #E_coli #Ecoli #Enterobacteriaceae #Coliform #lactoss_fermentation #gramstain #agar #microbiologia #microbiology #pharmaceuticals #Elmicrobiologist #lab #Science #Biology

How to Identify Gram Negative Species

Using On Farm Culture to Improve Mastitis Treatment
Episode #10: How to Identify Gram Negative Species

Gram negative organisms cannot be differentiated at the genus level (such as E. coli, Klebsiella or Enterobacter) on the agar plates used in on-farm cultures. However, they can be identified as lactose negative or lactose positive by what color they ferment lactose in MacConkey agar. In this episode, Dr. Pamela explains what this means and discusses treatment options for a gram negative infection. Hint: gram negative infections often resolve on their own. Therefore, it is not always necessary to treat with antibiotics. Remember, it is always advisable to consult your local veterinarian when making these decisions.

pseudomonas aeruginosa colony morphology on MacConkey agar-Blood agar(clear explain)

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I'm medical laboratory scientist.This video has information about pseudomonas aeruginosa colony morphology on MacConkey agar-Blood agar(clear explain).

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-pseudomonas aeruginosa colony morphology on Blood agar

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Gram Negative Bacteraemia

Speaker: Dr Susan Hopkins
Consultant in Infectious Diseases & Microbiology, Royal Free London NHS Foundation Trust, Healthcare Epidemiologist, Public Health England, Honorary Senior Lecturer, University College London

Gram Negative Rods (Lactose fermenters) escherichia coli, Klebsiella - NBDE & USMLE

FREE occlusion document for NBDE:

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Pseudomonas Aeruginosa Infection, And Treatment (Antibiotic)

Pseudomonas aeruginosa is a metabolically versatile ubiquitous gamma-proteobacterium that thrives in soil and aquatic habitats and colonizes the animate surfaces of plants, animals, and humans. P. aeruginosa may cause multiple infections in man that vary from local to systemic and from benign to life threatening. During the last few decades, the cosmopolitan Gram-negative bacterium has become one of the most frequent causative agents of nosocomial infections associated with substantial morbidity and mortality. Pneumonia and sepsis in intensive care unit (ICU) patients still have a bleak prognosis. Chronic airway infections with P. aeruginosa are a major cause of morbidity in people with cystic fibrosis (CF) or chronic obstructive pulmonary disease (COPD). Here we report on recent advances in the understanding of host–pathogen interactions with particular emphasis on infections with P. aeruginosa in humans.

Klockgether J, Tümmler B. Recent advances in understanding Pseudomonas aeruginosa as a pathogen. F1000Res. 2017;6:1261. Published 2017 Jul 28. doi:10.12688/f1000research.10506.1


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2053: Escherichia Coli or Pseudomonas Aeruginosa

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Pseudomonas aeruginosa

Pseudomonas aeruginosa
Instructional Tutorial Video

Pseudomonas aeruginosa Infections

Pseudomonas aeruginosa is a Gram-negative, aerobic, rod shaped bacteria. It is a common microorganism that can cause disease in animals, including humans. It is also the cause of various plant diseases.

It can be found in soil, water, skin flora and many man-made environments. It has the ability to utilize a wide range of organic material for food. In humans and animals this versatility enables the microorganism to infect damaged tissues and infect those with weakened immune systems.

Pseudomonas aeruginosa resistant to Tachyplesin I - Video Abstract ID 226687

Video abstract of original research paper “Transcriptome Analysis Reveals the Resistance Mechanism of Pseudomonas aeruginosa to Tachyplesin I” published in the open access journal Infection and Drug Resistance by Hong J, Jiang H, Hu J, et al.

Background: Tachyplesin I is a cationic antimicrobial peptide with a typical cyclic antiparallel β-sheet structure. We previously demonstrated that long-term continuous exposure to increased concentration of tachyplesin I can induce resistant Gram-negative bacteria. However, no significant information is available about the resistance mechanism of
Pseudomonas aeruginosa (P. aeruginosa) to tachyplesin I.
Materials and Methods: In this study, the global gene expression profiling of P. aeruginosa strain PA-99 and P. aeruginosa CGMCC1.2620 (PA1.2620) was conducted using transcriptome sequencing. For this purpose, outer membrane permeability and outer membrane proteins (OMPs) were further analyzed.
Results: Transcriptome sequencing detected 672 upregulated and 787 downregulated genes, covering Clusters of Orthologous Groups (COGs) of P. aeruginosa strain PA-99 compared with PA1.2620. Totally, 749 differentially expressed genes (DEGs) were assigned to 98 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, and among them, a two-component regulatory system, a beta-lactam resistance system, etc. were involved in some
known genes resistant to drugs. Additionally, we further attempted to indicate whether the resistance mechanism of P. aeruginosa to tachyplesin I was associated with the changes of outer membrane permeability and OMPs.
Conclusion: Our results indicated that P. aeruginosa resistant to tachyplesin I was mainly related to reduced entry of tachyplesin I into the bacterial cell due to overexpression of efflux pump, in addition to a decrease of outer membrane permeability. Our findings were also validated by pathway enrichment analysis and quantitative reverse transcription polymerase chain reaction (RT-qPCR). This study may provide a promising guidance for understanding
the resistance mechanism of P. aeruginosa to tachyplesin I.

Read the full article here:

Pseudomonas aeruginosa : Medical Microbiology

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Pseudomonas aeruginosa is a common gram-negative rod-shaped bacterium that can cause disease in plants and animals, including humans. A species of considerable medical importance, P. aeruginosa is a prototypical multidrug resistant (MDR) pathogen recognised for its ubiquity, its intrinsically advanced antibiotic resistance mechanisms, and its association with serious illnesses – especially nosocomial infections such as ventilator-associated pneumonia and various sepsis syndromes.
The organism is considered opportunistic insofar as serious infection is often superimposed upon acute or chronic morbidity – most notably cystic fibrosis and traumatic burns – or found in immunocompromised individuals, but the organism does produce a range of clinically important infections in the immunocompetent and/or in situations where no pre-existing vulnerability is required e.g. hot tub folliculitis. In all infections produced by P. aeruginosa, treatment is dually complicated by the organism's resistance profile, which may lead to treatment failure and/or expose patients to untoward adverse effects from advanced antibiotic drug regimens. This dilemma is a central clinical problem in the field of antimicrobial resistance.
It is citrate, catalase, and oxidase positive. It is found in soil, water, skin flora, and most man-made environments throughout the world. It thrives not only in normal atmospheres, but also in hypoxic atmospheres, thus has colonized many natural and artificial environments. It uses a wide range of organic material for food; in animals, its versatility enables the organism to infect damaged tissues or those with reduced immunity. The symptoms of such infections are generalized inflammation and sepsis. If such colonizations occur in critical body organs, such as the lungs, the urinary tract, and kidneys, the results can be fatal.[1] Because it thrives on moist surfaces, this bacterium is also found on and in medical equipment, including catheters, causing cross-infections in hospitals and clinics. It is implicated in hot-tub rash. It is also able to decompose hydrocarbons and has been used to break down tarballs and oil from oil spills.[2] P. aeruginosa is not extremely virulent in comparison with other major pathogenic bacteria species – for example Staphylococcus aureus and Streptococcus pyogenes – and does not fare especially well under suboptimal atmospheric conditions nor aggregate into enduring biofilms.

Phagocytosis of P. aeruginosa by neutrophil in patient with bloodstream infection (Gram stain)

Bacteriology- Gram Negative Bacilli (Part1)

In this video we are going to look at E. coli, Klebsiella pneumoniae, Proteus spp., Salmonella enterica and Shigella (Serogroups A, B, C, and D).

Microbiology Gram Negative Bacteria

This is the final lecture on Microbiology for Critical Care. We cover the medically important Gram negative bacteria.

Gram-Negative Solution: Lipopolysaccharide & Bacterial Structure – Microbiology | Lecturio

This video “Gram-Negative Solution: Lipopolysaccharide & Bacterial Structure” is part of the Lecturio course “Microbiology” ► WATCH the complete course on

- Gram-negative solution
- Thin murein layer
- Outer Membrane / Second membrane
- Parts of the lipopolysaccharide
- Lipid A or endotoxin
- Core polysaccharide
- O-antigen
- E. coli O157:H7
- The process how molecues get in
- Larger hydrophilic compounds
- Bacterial structure
- Periplasm
- Other exterior structures
- Fimbriae
- Capsule
- Flagellum
- Pili

Your lecturer is Prof. Dr. Vincent Racaniello. He is teaching microbiology and immunology at Columbia University in New York City. He is a leading expert in the research of viruses and human diseases. Therefore Racaniello has served on the editorial boards of scientific journals, such as the Journal of Virology or PLOS Pathogens. Furthermore he was the 2015 president of the American Society for Virology. Beyond that he is editor of an online virology blog and co-producer of the podcasts Netcast This Week in Virology, This Week in Parasitism and This Week in Microbiology.

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Pseudomonas Aeruginosa for the USMLE Step 1

Pseudomonas Aeruginosa for the USMLE Step 1, clear as crystal.

In this video we cover the unique features of the gram-negative bacterium known as Pseudomonas aeruginosa. We combine our novel image mnemonic style with conceptual components such as lab tests and gram stain images. Some of the features discussed include production of a grape-like fruity odor, production of a blue-green pigment known as pyocyanin, and a toxin known as phospholipase C. Enjoy!

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